Muhammad T. Islam*, Leticia Streck, Arnobio A. da Silva-Junior and Ana A. de Carvalho Melo-Cavalcante Pages 48 - 53 ( 6 )
Background: Praziquantel (Prz)-induced membrane permeability causes cell death of organisms. It may have antioxidative/pro-oxidative capacity. The aim of this study was to prepare Prz nanoemulsion and evaluate antioxidant capacity.
Materials and Methods: Radical scavenging capacity was checked by 1,1-dipheny-picrylhydrazyl (DPPH), azino-bisethylbenzthiazoline-sulfonic acid (ABTS); hydroxyl (OH) and nitrite oxide (NO) radical assays, while lipid peroxidation was done by thiobarbitiric acid substances (TBARS), reduction potential (RP) by ferric reduction, and membrane protection by hemolysis (HL) inhibition test in rat erythrocytes taking Trolox (TRO), an α-tocopherol analogue as a positive control. Additionally, an oxidative/antioxidative potential of PrzNE was checked in a wild type (SOD-WT) and deficient (SOD1Δ, SOD2Δ, CAT1Δ, SOD1ΔSOD2Δ and SOD1ΔCAT1Δ) Saccharomyces cerevisiae strains using hydrogen peroxide (H2O2) as a stressor.
Results: PrzNE, in a concentration-dependent manner, scavenged free radicals. It significantly (p <0.05) scavenged NO and OH radicals. PrzNE, alone induced slight oxidative stress, while in cotreatment, it significantly (p <0.05) protected S. cerevisiae strains from the damaging effect of H2O2.
Conclusion: The OH and NO radical scavenging capacity may be linked to the protective effect of the RBCs membrane and S. cerevisiae cells. PrzNE exerted a moderate antioxidative capacity.
Antioxidant, nanoemulsion, praziquantel, Rattus norvegicus, Saccharomyces cerevisiae.
Laboratory of Genetic Toxicology, Federal University of Piauí, Teresina (Paiui)-64009-550, Departament of Pharmacy, Federal Uuniversity of Rio Grande do Norte, Natal-RN-59012- 570, Departament of Pharmacy, Federal Uuniversity of Rio Grande do Norte, Natal-RN-59012- 570, Laboratory of Genetic Toxicology, Federal University of Piauí, Teresina (Paiui)-64009-550