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Non-Clinical Screening of Cyto-Genotoxicologic Status of Praziquantel Nanoemulsion

[ Vol. 14 , Issue. 1 ]

Author(s):

Muhammad T. Islam*, Leticia Streck, Samara W.C. Silva, Ana M.O.F. da Mata, Marcus V.O.B. de Alencar, Ricardo M. de Carvalho, Arnobio A. da Silva-Junior and Ana A.C. Melo-Cavalcante   Pages 33 - 38 ( 6 )

Abstract:


Background: Prazequantel (Prz) is an important medication in humans and animals. It acts against a number of pathogens. In this study, Prz nanoemulsion (PrzNE) was prepared and evaluated for toxic, cytotoxic and mutagenic potentials.

Materials and Methods: The cytotoxic effect of PrzNE was observed in Artemia salina and Allium cepa, while toxicity and mutegenicity in A. cepa test system. Potassium di-chromate (K2Cr2O7) and copper sulphate (CuSO4.5H2O) were taken as standards for A. salina and A. cepa test, respectively.

Results: PrzNE at 16 µM caused 100% mortality of the brine shripms. It exerted a concentrationdependent toxic and cytotoxic effects in A. cepa and A. salina. In comparison to the CuSO4.5H2O, PrzNE produced less chromosomal aberrations (CA), where chromosomal fragments/loose and bridges were most frequently observed.

Conclusion: PrzNE may readily cross the cell membranes of A. cepa and A. salina, increase the permeability of the cell membrane and/or exert an oxidative stress, thus causes a cytotoxic effect. The PrzNE was more cytotoxic than mutagenic in nature.

Keywords:

Cytotoxicity, mutagenicity, praziquantel nanoemulsion, enzymes, hypotension, budding parenchyma.

Affiliation:

Department for Management of Science and Technology Development, Ton Duc Thang University, Ho Chi Minh City, 700000, Departament of Pharmacy, Federal Uuniversity of Rio Grande do Norte, Natal-RN-59012-570, Postgraduate Program in Biotechnology, Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina( Paiui)-64009-550, Postgraduate Program in Biotechnology, Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina( Paiui)-64009-550, Postgraduate Program in Biotechnology, Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina( Paiui)-64009-550, Postgraduate Program in Biotechnology, Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina( Paiui)-64009-550, Departament of Pharmacy, Federal Uuniversity of Rio Grande do Norte, Natal-RN-59012-570, Postgraduate Program in Biotechnology, Northeast Biotechnology Network (RENORBIO), Federal University of Piauí, Teresina( Paiui)-64009-550

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