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Effect of Variable Doses of Paederia foetida L. Combat Against Experimentally- Induced Systemic and Topical Inflammation in Wistar Rats

[ Vol. 14 , Issue. 1 ]

Author(s):

Vikas Kumar*, Gaurav Kaithwas, Firoz Anwar, Mahfoozur Rahman, Dinesh K. Patel, Yashwant Singh and Amita Verma   Pages 70 - 79 ( 10 )

Abstract:


Background: According to the Indian traditional system of medicine, Paederia foetida L. (Rubiaceae) is considered as the most valuable drug for numerous protective effects against different types of metabolic diseases and disorders such as inflammation, arthritis, pain, etc. To date, no study has been undertaken to scrutinize the systemic and topical anti-inflammatory effect of Paederia Foetida L. The present study is an attempt to scrutinize systemic and topical anti-inflammatory effect of Paederia foetida L and its possible mechanism of action.

Materials and Methods: In vitro antioxidant studies were performed via hydrogen peroxide (H2O2), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), nitric acid (NO) and superoxide model for free radical scavenging potential. Oral and topical anti-inflammatory activity of Paederia foetida L. methanolic extract was experimented through carrageenan, egg albumin, serotonin, histamine, xylene, Arachidonic acid (AA) and 12-O-tetradecanoylphorbol-acetate (TPA) established methods.

Result & Discussion: PF extract confirmed the presence of total phenolic phytoconstituents in the PF extract. Invitro antioxidant studies confirmed that the PF extract scavenges the free radical in various models. On the other hand, PF extract significantly reduces the carrageenan-induced inflammation by inhibiting the synthesis of prostaglandin (PGE2); egg-induced inflammation via reduction in secretion of serotonin and histamine. Topical inflammation induced by the xylene, TPA and AA, was subsided significantly (P<0.001) by the PF extract in a dose-dependent manner via inhibition of PGE2, 5- lipooxygenae and cyclo-oxygenase.

Conclusion: We can now conclude that PF extract inhibits the systemic and topical inflammation in dosedependent manner. The PF extract probably inhibited the synthesis of PGE2, 5-lipooxygenae and cyclooxygenase.

Keywords:

Paederia foetida L., carrageenan, histamine, serotonin, prostaglandin, 5-lipooxygenae, cyclo-oxygenase.

Affiliation:

Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad-211007, Uttar Pradesh, Department of Pharmaceutical Sciences, School of Biosciences and Biotechnology, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow 226025, Uttar Pradesh, Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad-211007, Uttar Pradesh, Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad-211007, Uttar Pradesh, Pharmacokinetics & Metabolism Division, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, Bio-organic and Medicinal Chemistry Research Laboratory, Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad -211007, Uttar Pradesh

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