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Antioxidant, Anti-inflammatory, Analgesic, and Antipyretic Effects of Urospermum dalechampii (L.) Scop. ex F.W.Schmidt

[ Vol. 14 , Issue. 3 ]

Author(s):

Wafa Bouzid*, Mouloud Yahia, Naima Benkiki, Messaoud Hachemi, Asma Meziti and Hamada Haba   Pages 264 - 272 ( 9 )

Abstract:


Objective: In the present work, we aim to investigate the acute toxicity and to evaluate the antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the methanol extract (UDME) of Urospermum dalechampii (L.) Scop. ex F.W.Schmidt.

Methods: Total phenolics, total flavonoids, and condensed tannins content were estimated by colorimetric methods. The antioxidant activity was evaluated by various antioxidant assays including DPPH free radical scavenging, β-carotene bleaching test, and metal chelating ability. The acute oral toxicity study was conducted where the limit test dose of 2000 mg/kg body weight was used. The analgesic activity was estimated peripherally using the writhing test and the anti-inflammatory activity was established with the carrageenan-induced pleurisy model. Moreover, the antipyretic activity was estimated using brewer's yeast-induced hyperthermia assay in rats.

Results: Total phenolic content determination showed that UDME is rich in phenolic compounds and it exhibited a good antioxidant activity. A single dose of the extract did not cause any signs of acute toxicity in rats. Significant antinociceptive effect (68.4 and 80.6%) was found in the writhing test at the doses of 200 and 400 mg/kg, p.o. respectively. Also, both doses significantly decreased the neutrophil migration to the pleural cavity. Furthermore, no antipyretic activity was observed.

Conclusion: This study demonstrates that the aerial parts of Urospermum dalechampii exhibited potent antioxidant, anti-inflammatory, and analgesic activities which proved the use of this species in folk medicine.

Keywords:

Urospermum dalechampii (L.) Scop. ex F.W.Schmidt, antioxidant, phenolic compounds, acute toxicity, analgesic, anti-inflammatory.

Affiliation:

Biotechnology`s Laboratory of the Bioactive Molecules and the Cellular Physiopathology, Faculty of the Sciences of Nature and Life, University of Batna 2, Batna, Biotechnology`s Laboratory of the Bioactive Molecules and the Cellular Physiopathology, Faculty of the Sciences of Nature and Life, University of Batna 2, Batna, Biotechnology`s Laboratory of the Bioactive Molecules and the Cellular Physiopathology, Faculty of the Sciences of Nature and Life, University of Batna 2, Batna, Biotechnology`s Laboratory of the Bioactive Molecules and the Cellular Physiopathology, Faculty of the Sciences of Nature and Life, University of Batna 2, Batna, Laboratory of the Characterization and the Development of Natural Resources, Faculty of the Sciences of Nature and Life, University of Mohamed El Bachir El Ibrahimi, Bordj Bou Arreridj, Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Matter Sciences, Batna 1 University, Batna

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