Ahmed A. Haroun*, Hala A. Amin and Sameh H. Abd El-Alim Pages 364 - 372 ( 9 )
Backgroung: Using of multi-walled carbon nanotubes (MWCNTs) as a drug delivery system, can avoid the need for solvents and preventing drug precipitation in aqueous solution. Soyasapogenol B (SSB) acts as therapeutic agent owing to itsimportance. Hence, this work deals with preparation and characterization of SBB loaded onto functionalized MWCNTs with tetraethyl orthosilicate (TEOS) and/or chitosan.
Methods: Soyasapogenol B (SSB) was immobilized onto functionalized MWCNTs using miniemulsion technique. Moreover, niosomes were utilized to encapsulate the prepared systems. The formulations were analyzed by Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM) and particle size distribution analysis. In vitro release profiles of loaded SSB particles were carried out and kinetics of release were also studiesd. In vitro cytotoxicity of the prepared materials was examined and evaluated by SRB assay using different human cell lines such as normal melanocytes (HFB4), and carcinoma breast and liver (MCF7 and HepG2) in comparison with the standard doxorubicin.
Results: SSB exihibited successful encapsulation of the SSB MWCNTs, resulting in an increase in drug release. Study of kinetics of release revealed presence of several complex factors affecting SSB release. Mathematical processing of the in vitro release data showed that the release of SSB from niosomal formulations obeyed more than one model. The second order and Higuchi’s models were the most fitting models in case of presence chitosan or TEOS, respectively. while, all formulations exhibited low cytotoxic properties on all tested celle lines.
Conclusion: FTIR, particle size and TEM analysis confirmed that SSB was successful loaded onto functionalized MWCNTs. Moreover, the different nioome formulations based on functionalized MWCNTs were prepared with sustainable SSB release in aqueous medium. The cytotoxicity could be minimized in case of chitosan and TEOS functionalization.
Multi-walled carbon nanotubes, TEOS, chitosan, in vitro release kinetics, soyasapogenol B, in vitro cytotoxicity.
Chemical Ind. Res. Div., National Research Centre, 33 Bohouth Str., Dokki, Giza, Chem of Natural & Microbial Products Dept, National Research Centre, 33 Bohouth Str., Dokki, Giza, Pharmaceutical Technol Dept, National Research Centre, 33 Bohouth Str., Dokki, Giza