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Synthesis, Characterization and Antifungal Activity of N-Methyl Chitosan and Its Application on the Gauze

[ Vol. 14 , Issue. 4 ]

Author(s):

Mardiyah Kurniasih*, Ratna S. Dewi, Purwati Purwati, Dadan Hermawan and Hassan Y. Aboul-Enein*   Pages 347 - 356 ( 10 )

Abstract:


Background: Chitosan is a natural polymer which has limited solubility. Chitosan is capable of being modified using multiple alternatives for chitosan chemical modifications that provide different desirable properties.

Method: N-methyl chitosan was successfully prepared and characterized. N-methyl chitosan was characterized by the FTIR, XRD, SEM, TEM, solubility, water content, and specific surface area. Antifungal activity against Candida albicans of N-methyl chitosan and gauze coated N-methyl Chitosan were also determined in this study. The antifungal activity tests were performed using agar diffusion and broth microdilution methods. Gauze coating with N-methyl chitosan was performed with a pad-dry-cure method. The SEM photograph of N-methyl chitosan clearly showed the rugged nature of particles. The TEM image shows that N-methyl chitosan produced from synthesis possessed round shape, with an average diameter of 50 nm.

Results: The results of solubility in water, water content, and specific surface of N-methyl chitosan were 2.04 mg/mL, 18.12%, and 0.995 m /g, respectively.

Conclusion: N-methyl chitosan exhibited antifungal activity against C. albicans with MIC and MFC 0.75% and 1%, respectively. The maximum clear zone produced by gauze coated N-methyl chitosan occurred at 1% concentration of N-methyl chitosan, 60 seconds of dipping time, and 10 times of dipping frequency.

Keywords:

Chitosan, N-methyl chitosan, gauzed, antifungal activity, C. albicans, respiratory tract.

Affiliation:

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto 53123, Mycology & Phytopathology Laboratory, Faculty of Biology, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto 53123, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto 53123, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno, Purwokerto 53123, Department of Pharmaceutical and Medicinal Chemistry, Pharmaceutical and Drug Industries, Research Division, National Research Centre, Dokki, Giza 12622

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