Moni P. J. Kizhakedathil and Subathra Devi Chandrasekaran* Pages 387 - 396 ( 10 )
Background: Rhizosphere is rich in microbial flora. Actinomycetes are a diverse group of organisms which are reported to produce antibiotics, vitamins, enzymes and plant growth promoting substances.
Aim: The main objective of this study was to screen actinomycetes from the soil samples for the production of various enzymes that could be used for industrial applications.
Method: Rhizosphere soils samples were collected from five different agricultural fields located in the Kolathur village, Tamil nadu, India. Thirty isolates were isolated and designated as VITMS1-VITMS30. The maximum number of isolates (43.3 %) was obtained from the rhizosphere soil samples of brinjal field and maximum number of isolates was isolated on starch casein agar (90%). The isolates were characterized morphologically in ISP media 1-7, Starch casein agar, Bennet agar and AIA, respectively.
Results: The isolates were screened for eight different enzymes - α-amylase, protease, gelatinase, chitinase, lipase, DNase, cellulase and urease. From the results observed, all the thirty isolates showed positive α-amylase, lipase and gelatinase activity. All the isolates exhibited cellulase activity but in very low levels. Out of 30 isolates, 19 isolates exhibited protease activity; 24 isolates were positive for DNase activity; 10 isolates showed chitinase activity and 20 isolates showed urease activity.
Conclusion: The soil samples from the kolathur village harbor diverse actinomycetes which exhibited good enzymatic activity.
Actinomycetes, rhizosphere soil, enzymes, amylase, gelatinase, lipase.
Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore – 632014, Tamil Nadu, Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore – 632014, Tamil