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Phytochemical Profile, Antimicrobial and Antioxidant Activities of Cistus clusii Essential oil From Algeria

[ Vol. 16 , Issue. 2 ]

Author(s):

Souad Khebri, Naima Benkiki, Mouloud Yahia and Hamada Haba*   Pages 109 - 114 ( 6 )

Abstract:


Objective: In this work, we investigated the chemical composition and the antioxidant and antimicrobial activities of the Algerian Cistus clusii Dunal essential oil.

Methods: Cistus clusii essential oil has been extracted using hydrodistillation method and characterized by GC/MS. The antioxidant activity was evaluated by two different methods DPPH free radical scavenging, and β-carotene bleaching test. The antimicrobial activity was carried out using disc diffusion method against reference strains; Pseudomonas aeruginosa (ATCC 27865), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), clinical bacterial (Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus) and fungal (Candida albicans) isolates.

Results: The chemical profile of the oil showed 31 compounds. This profile was dominated by the presence of monoterpene hydrocarbons (78.4%), oxygenated derivatives (18.8%) and sesquiterpene hydrocarbons as minor constituents of this essential oil (1.9%). The major constituents were Camphene (20.6%), γ-Terpinene (16.5%), Sabinene (12.8%), and Terpineol-4 (13.5%). Other compounds were found in moderately good amounts like α-Terpinene (8.9%), α-Thujene (5.9%), α-Pinene (4.2%), and α- Terpinolene (3.1%). The results of the antimicrobial activity showed that the highest activity was observed against C. albicans and it was higher than positive control Fluconazole. The essential oil exhibited a strong antioxidant activity, especially in the β-carotene system.

Conclusion: This study indicates that Cistus clusii essential oil showed potent antioxidant and moderate antimicrobial activities which could be used to explain the use of this species in traditional medicine.

Keywords:

Cistus clusii, GC/MS, Candida albicans, clinical strains, essential oil, antibacterial agents.

Affiliation:

Biotechnology’s Laboratory of the Bioactive Molecules and the Cellular Physiopathology, Faculty of the Sciences of Nature and Life, University of Batna-2, Batna, Biotechnology’s Laboratory of the Bioactive Molecules and the Cellular Physiopathology, Faculty of the Sciences of Nature and Life, University of Batna-2, Batna, Biotechnology’s Laboratory of the Bioactive Molecules and the Cellular Physiopathology, Faculty of the Sciences of Nature and Life, University of Batna-2, Batna, Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Matter Sciences, Batna-1 University, Batna

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