George Laylson da Silva Oliveira, Maria das Dores Alves de Oliveira, Maria da Conceicao Oliveira Prado, Alexandre de Barros Falcao Ferraz, Jose Carlos Correia Lima da Silva Filho, Joaquim S. da Costa Junior and Ana Paula dos S. C. Lima da Silva* Pages 284 - 293 ( 10 )
Background:Garcinielliptone FC corresponds to a polyprenylatedacylphloroglucinol having a benzophenonic core (diphenylmethanone) substituted with isoprenyl(s) group(s) (3-methyl-2-butenyl) and 2-isopropenyl-hex-5-enyl.Objectives:The present work evaluated the antioxidant activity of garcinielliptone FC (GFC) in vitroagainst non-biological radicals [2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+)] and ex vivo against oxidative damage induced byAAPH (2,2'-azobis-2-methylpropionamidine dihydrochloride) and iron/citrate ion in erythrocytes and mitochondria, respectively. Methods: In addition to the protective effect, the main biochemical indexes of oxidative stress, such as lipid peroxidation through the formation of Thiobarbituric Acid Reactive Substances (TBARS), Super-oxide Dismutase (SOD), Catalase (CAT) activity and reduced glutathione (GSH) levels. Results:According to the results obtained in erythrocytes, the antioxidant results at concentrations of 0.1, 0.3, 0.7, 1.5 and 3.0 mM were 26.34 ± 0.68, 43.39 ± 2.17, 62.27 ± 2.17, 86.69 ± 0.47 and 92.89 ± 0.45%, respectively, where GFC reduced the rate of oxidative hemolysis when compared to AAPH (p<0.05). The antioxidant activity observed in erythrocytes was also seen in mitochondria in which GFC reduced mitochondrial swelling by increasing the absorbance when compared to iron/citrate ion com-plex (p<0.05). In both biological models, GFC had an antioxidant effect on erythrocyte and mitochon-drial redox balance when analyzing oxidative stress biomarkers, such as reduction of lipid peroxidation and inhibition of depletion in the activity of SOD, CAT and GSH levels. Conclusion: In conclusion, GFC had in vitro and ex vivo antioxidant activity against oxidative damage induced in erythrocytes and mitochondria acting on the erythrocytic and mitochondrial redox balance.
Antioxidant, erythrocytes, garcinielliptone FC, mitochondria, radicals
Department of Biology, Federal Institute of Mato Grosso, Guarantã do Norte - MT, CEP 78520-000, Guarantã do Norte Campus, Department of Chemistry, Federal Institute of Piaui, Teresina, CEP 64000-040, Piaui, Department of Biology, Federal University of Piaui, Teresina, CEP 64049-550, Piaui, Institute of Chemistry, Lutheran University of Brazil, CEP 92425900, - Canoas, Rio Grande do Sul, Department of Computer Science, Federal University of Piaui, Teresina, CEP 64049-550, Piaui, Department of Chemistry, Federal Institute of Piaui, Teresina, CEP 64000-040, Piaui, Department of Pharmacy, Federal University of Piaui, Teresina, CEP 64049-550, Piaui