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β-Sitosterol Oxides From the Dried Stem of Salix gilgiana Inhibit the Proliferation of HL-60 Leukemic Cells

[ Vol. 16 , Issue. 3 ]

Author(s):

Manami Oyama, Tetsuo Tokiwano, Hiromi Ota, Kouichi Mizuno, Keimei Oh, Satoru Kawaii and Yuko Yoshizawa*   Pages 329 - 334 ( 6 )

Abstract:


Background:Salix gilgianais a deciduous tree that grows in northern Japan, the Korean peninsula, eastern Russia along the Ussuri River, and northeast China. The stem of this tree is dried and consumed orally as a folk medicine. Our intensive screening of various plant materials found that the MeOH extract of its dried stem exhibited significant antiproliferative activity against HL-60 leukemic cells with an IC50of 16 ppm. We systematically investigated the biologically active compounds of the MeOH extract of the dried stem of S. gilgiana.

Methods:The MeOH extract of S. gilgianadried stem was fractionated by a repeated chromatography monitored by antiproliferative activity against HL-60 leukemic cells. Five active compounds were iso-lated and the structures were elucidated by MS, 1H-and 13C-NMR spectroscopy, and X-ray analysis.

Results:The active compounds were identified as 7-ketositosterol (I), 7β-hydroxysitosterol (II), 7α-hydroxysitosterol(III), (4-hydroxyphenyl)ethanol (IV), and (4-hydroxyphenyl)propan-1-ol (V). The strongest activity was found for 7α-hydroxysitosterol (III), with an IC50of 8.4 μM. This is the first report of the isolation of these compounds fromS. gilgiana.

Conclusion: Five compounds were isolated by a repeated chromatography under the guidance of anti-proliferative bioassay using HL-60. The structures were identified as three β-sitosterol oxides and two phenoliccompounds. Since Salixspecies, namely, willow trees, havebeneficial characteristics including rapid growth, easy cloning, and resistance to high humidity and dryness, they may be utilized as a rela-tively inexpensive tool for the efficient production of useful bioactive materials..

Keywords:

Salix gilgiana, HL-60, antiproliferative activity, 7-ketositosterol, salicin, bovine serum.

Affiliation:

Laboratory of Bio-organic Chemistry, Akita Prefectural University, Akita 010-0195, Laboratory of Bio-organic Chemistry, Akita Prefectural University, Akita 010-0195, Division of Instrumental Analysis, Advanced Science Research Center, Okayama University, Okayama 700-8530, Laboratory of Bio-organic Chemistry, Akita Prefectural University, Akita 010-0195, Laboratory of Bio-organic Chemistry, Akita Prefectural University, Akita 010-0195, Bio-organic Chemistry, Tokyo Denki University, Hatoyama, Saitama 350-0394, Laboratory of Bio-organic Chemistry, Akita Prefectural University, Akita 010-0195



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