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Antiproliferative Properties of Extracts from Stachys sieboldii MIQ

[ Vol. 16 , Issue. 3 ]

Author(s):

Eun Na, Jung Woo Lee, Stefan Winkler and Sun Young Lim*   Pages 342 - 347 ( 6 )

Abstract:


Background:The objective of this study was toinvestigatetheantiproliferativeeffectofStachyssieboldiiMIQ.extractson cancer cell lines.

Methods: DriedS. sieboldiiMIQ.was extractedusingacetone/methylenechloride(A+M) or methanol(MeOH), and thenfractionatedusingn-hexane,85%aq.methanol(MeOH),butanol(BuOH)anddis-tilledwater.ThecytotoxicactivityofS. sieboldiiMIQ.againstAGShumangastric,HT-29humancolonandHT-1080 fibroblastcancercelllineswas assessed using the3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)assay.

Results:The A+Mextractshowedsignificantlyhigherinhibition of cell growth in all three cell lines comparedto the MeOHextract(p <0.05). AlltheextractsandfractionsfromS. sieboldiiMIQ.de-creasedthegrowthofAGScells,andtheeffectwasconcentration-dependent.Amongthedifferentfrac-tions,then-Hexane and 85% aq. MeOH fractions inhibited AGS cell growth by >50%at a concentra-tion of0.1 mg/mL (p <0.05).All fractions inhibited the proliferation of HT-29 cancer cell lines (p <0.05), showing >50% inhibition at a concentration of 0.25 mg/mL. TheA+Mextract inhibited thegrowthoftheHT-1080cancercellsby 60%at a concentrationof0.25 mg/mL. Then-Hexane and 85%aq.MeOHfractions showedthehighest growth inhibitoryeffectontheHT-1080cancercells(p <0.05), similarto that observedinthe AGS cancercells.

Conclusion: Thus,then-Hexane and 85%aq.MeOHfractions fromS. sieboldiiMIQ. likelycontainbioactivecompoundssuchaspolyphenolsandflavonoids that could prevent cancer proliferation. Fur-ther research is needed to isolatetheseimportantcompoundsfromtheextracts.

Keywords:

AGSgastric, Antiproliferation, HT-29colon, HT-1080fibroblast, StachyssieboldiiMIQ

Affiliation:

Ocean Science and Technology School, Korea Maritime & Ocean University, Busan, Division of Marine Bio-science, Korea Maritime & Ocean University, Busan, Institute of Applied Cell Culture, Technische Universit├Ąt Munich, Munich, Division of Marine Bioscience, Korea Maritime and Ocean University, Busan



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