Varsha Yadav, Neha Kapoor*, Soma M. Ghorai and Pradeep Pages 449 - 459 ( 11 )
Background: Biosynthesis of nanoparticles from aqueous leaf extract of ‘Selaginella bryop-teris’ is a green chemistry approach and is considered to be one of the most efficient methods as it is devoid of toxic chemicals as well as provides natural capping agents for the stabilization of synthesized nanoparticles. ‘S.bryopteris’ also known as ‘Sanjeevani’ (in India), is thought to be prospective natural resource that possesses extraordinary pharmaceutical potential.Objective:S. bryopteris is exclusively native to India and has already been known for its expression of stress-associated genes and high levels of protective metabolites of sugars, phenolic compounds, and polyols. Its potential as an antibacterial agent is being elucidated. Methods: Different leaf extract volumes, silver nitrate (AgNO3) concentrations, and reaction time were investigated separately and the optimal conditions for the synthesis of AgNPs were suggested. The re-sulting AgNPs were characterized by various techniques like Ultraviolet-Visible (UV-Vis) Spectros-copy, Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and X-Ray Diffraction (XRD). Antibacterial assays were carried out by using both agar disk and well diffusion method. Result: The AgNPs synthesized in this process were found to have efficient antimicrobial activity against both Gram-positive as well as Gram-negative bacteria. The antibacterial efficacy of S. bryopteriswas consciously tried on uropathogenic Escherichia coli (Gram-negative bacteria) and Bacillusmegaterium(Gram-positive bacteria) which have the self-limiting food poisoning potential along with opportunistic uropathogenic bacterial strains namely Proteus mirabilis (Gram-negative bacteria) and a non-pathogenic Micrococcus luteus (Gram-positive bacteria) for comparison. Conclusion:S. bryopteris mediated silver nanoparticles’ synthesis is attempted for being cost-effective, eco-friendly and safe for human therapeutics.
Biomimetic, silver nanoparticles, phytochemicals, antimicrobial activity, natural product, therapeutics.
Department of Zoology, Hindu College, University of Delhi, Delhi-110007, Department of Chemistry, Hindu College, University of Delhi, Delhi-110007, Department of Zoology, Hindu College, University of Delhi, Delhi-110007, Department of Chemistry, Hindu College, University of Delhi, Delhi-110007