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3,6-dichloro-1,2,4,5-Tetrazine Assayed at High Doses in the Metastatic Breast Cancer Cell Line MDA-MB-231 Reduces Cell Numbers and Induces Apoptosis

[ Vol. 16 , Issue. 4 ]

Author(s):

Mohamed Abdelkarim*, Khaoula Ben Younes, Ines Limam, Refka Guermazi, Amel Ben Ammaar El Gaaied and Fatma Ben Aissa-Fennira   Pages 546 - 550 ( 5 )

Abstract:


Background: The lack of treatment options for patients with chemotherapy-resistant cancers is pushing the field toward the development of new therapies.1,2,4,5-tetrazine derivatives are a class of heterocyclic compounds that exhibit a broad spectrum of antitumor activities.

Objective:The purpose of this study was to assess the biological activity of four s-tetrazine derivatives by substitution of two chloride atom of 3,6-dichloro-1,2,4,5-tetrazine with long hydrophobic side chains.

Method: We analyzed the anti-proliferative effects of four s-tetrazine derivatives with MTT assay and their pro-apoptotic effect with AV/ IP flow cytometry analysis and Hoechst 33342 staining.

Results: We demonstrated that 3,6-dichloro-1,2,4,5-tetrazine (compound (1)) has a cytotoxic effect and induces apoptosis.

Conclusion: 3,6-dichloro-1,2,4,5-tetrazine presents a new cytotoxic drug against metastatic breast cancer cell line MDA-MB-231 in vitro.

Keywords:

1, 2, 4, 5-tetrazine derivatives, in vitro experiment, MDA-MB-231 cell line, breast cancer, 3, 6-dichloro-1, 2, 4, 5-tetrazine, apoptosis

Affiliation:

Laboratory of Genetics Immunology and Humans Pathologies, Faculty of sciences of Tunis, Tunis El Manar University, Laboratory of Genetics Immunology and Humans Pathologies, Faculty of sciences of Tunis, Tunis El Manar University, Laboratory of Genetics Immunology and Humans Pathologies, Faculty of sciences of Tunis, Tunis El Manar University, Laboratory of Organic Molecular Chemistry, ENSIT MONTFLEURY 5 Taha Hussein Avenue 1008 Montfleury, University of Tunis,Tunis, Laboratory of Genetics Immunology and Humans Pathologies, Faculty of sciences of Tunis, Tunis El Manar University, Laboratory of Genetics Immunology and Humans Pathologies, Faculty of sciences of Tunis, Tunis El Manar University



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