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3,6-dichloro-1,2,4,5-Tetrazine Assayed at High Doses in the Metastatic Breast Cancer Cell Line MDA-MB-231 Reduces Cell Numbers and Induces Apoptosis

Author(s):

Mohamed Abdelkarim*, Khaoula Ben Younes, Ines Limam, Refka Guermazi, Amel Ben Ammaar El Gaaied and Fatma Ben Aissa-Fennira   Pages 1 - 5 ( 5 )

Abstract:


Background: The lack of treatment options for patients with chemotherapy-resistant cancers is pushing the field toward the development of new therapies. 1,2,4,5-tetrazine derivatives are a class of heterocyclic compounds that exhibit a broad spectrum of antitumor activities. Objective: The purpose of this study was to assess the biological activity of four s-tetrazine derivatives by substitution of two chloride atom of 3,6-dichloro-1,2,4,5-tetrazine with long hydrophobic side chains. Method: We analyzed the anti-proliferative effects of four s-tetrazine derivatives with MTT assay and their pro-apoptotic effect with AV/ IP flow cytometry analysis and Hoechst 33342 staining. Results: We demonstrated that 3,6-dichloro-1,2,4,5-tetrazine (compound (1)) has a cytotoxic effect and induces apoptosis. Conclusion: 3,6-dichloro-1,2,4,5-tetrazine presents a new cytotoxic drug against metastatic breast cancer cell line MDA-MB-231 in vitro.

Keywords:

5-tetrazine derivatives, in vitro experiment, MDA-MB-231 cell line, breast cancer, 3, 6-dichloro-1, 5-tetrazine, apoptosis

Affiliation:

Laboratory of onco-hematology, Faculty of medicine of Tunis, Tunis El Manar University, Tunis, Laboratory of onco-hematology, Faculty of medicine of Tunis, Tunis El Manar University, Tunis, Laboratory of onco-hematology, Faculty of medicine of Tunis, Tunis El Manar University, Tunis, Laboratory of Organic Molecular Chemistry, ENSIT MONTFLEURY 5 Taha Hussein Avenue 1008 Montfleury, University of Tunis, Tunis, Laboratory of genetics immunology and humans pathologies, Faculty of sciences of Tunis, Tunis El Manar University, Laboratory of onco-hematology, Faculty of medicine of Tunis, Tunis El Manar University, Tunis



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