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Isolation, Characterization and Hepatoprotective Activity of Naturally Occurring Protopine Against Simvastatin Induced Liver Toxicity in Experimental Rodents

Author(s):

Ramesh Kumar Gupta*, Sudhansu Ranjan Swain, Jagannath Sahoo, Sachin Chaudhary and Amresh Gupta   Pages 1 - 7 ( 7 )

Abstract:


Background: The present study is based on isolation, characterization and determination of in-vivo hepatoprotective effect of naturally occurring protopine from Hedyotis corymbosa.

Methods: In this project protopine was isolated from H. coryambosa by column chromatography method using chloroform: methanol: diethylamine (9:1:1) as the mobile phase and structural elucidation of compound was confirmed by the UV, FT-IR, 1H-NMR and 13C-NMR and mass spectroscopy, followed by determination of in-vivo liver protective effect of protopine employing simvastatin (20 mg/kg, p.o.), induced hepatotoxicity in experimental rodents. The liver protective activity was assessed by interpreting distinct biochemical parameters like SGOT, SGPT, cholesterol, urea, total bilirubin, total protein and albumin along with the haematological and histopathological studies.

Results: The different spectroscopic methods confirmed that the compound that was isolated from H. coryambosa is protopine, an isoquinoline alkaloid. The treatment with protopine significantly at (P<0.05-P<0.001) and dose-dependently reversed simvastatin induced elevated level of SGOT, SGPT, cholesterol, urea, total bilirubin and restored the total protein and albumin level. Furthermore, protopine also signify the blood parameters at dose of 11 and 22 mg/kg and restored the body defence mechanism. The histological examination revealed that protopine at dose of 22 mg/kg showed regeneration of hepatocytes around central vein with near normal liver architecture.

Conclusion: The results of this study exhibited liver protective effect of protopine against simvastatin induced liver injury and thereby scientifically support its traditional use.

Keywords:

Protopine, Hedyotis corymbosa, Serum glutamic oxaloacetic transaminase, Cholesterol, Simvastatin, Liver, Cholesterol

Affiliation:

Moradabad Educational Trust, Group of Institutions, Faculty of Pharmacy, Moradabad 244001, Uttar Pradesh, Moradabad Educational Trust, Group of Institutions, Faculty of Pharmacy, Moradabad 244001, Uttar Pradesh, School of Pharmacy, Krishna Institute of Engineering and Technology, Ghaziabad 201001, Uttar Pradesh, College of Pharmacy, University of Sharjah, Sharjah 27272, Goel Institute of Pharmacy and Sciences, Lucknow 226024, Uttar Pradesh



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