Rajesh P. Shastry* and Mohammed Aman Pages 887 - 891 ( 5 )
Background: Bacteria communicate by producing small signaling molecules to maintain threshold cell density. Quorum sensing inhibition is an effective and most potent approach for the prevention of cell to cell communication.Methods: In the present study, the methanol extract of Garcinia indica was tested for anti-quorum sensing activity using Chromobacterium violaceum as a biosensor. The extract inhibited violacein production in biosensor strain without affecting the growth of the bacteria and this was corroborated by bioautography assay. Results: The bioactive fraction was analysed by the aid of GC-MS revealed that, the presence of coumarin (7-hydroxy-3-(1,1-dimethylprop-2-enyl) coumarin) and eugenol (1,2-dimethoxy-4-(1-methoxy-1- propenyl) benzene) derivatives as anti-quorum sensing molecules. Conclusion: A detailed investigation is required to study the mechanism of action involved in control of quorum sensing signals and possible applications.
Anti-quorum sensing, coumarin, eugenol, G. indica, plant seed extract, biosensor.
Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore-575018, Department of Microbiology, School of Sciences, Jain (Deemed to be University), Jayanagar, Bangalore-560011