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GC-analysis, and Antioxidant, Anti-inflammatory, and Anticancer Activities of Some Extracts and Fractions of Linum usitatissimum

[ Vol. 16 , Issue. 9 ]

Author(s):

Eman A. Morsi, Hend O. Ahmed*, Heba Abdel-Hady, Mortada El-Sayed and Mohamed A. Shemis   Pages 1306 - 1318 ( 13 )

Abstract:


Context: Linum usitatissimum (Linn); known as Flaxseed, is one of the most important medicinal plants traditionally used for health benefits and also as nutritional purposes.

Objective: Estimation of total phenolic and flavonoid contents, and evaluation of the antioxidant, antiinflammatory and anticancer activities have been performed on hexane, ethyl acetate, n-butanol, and methanol extracts and also on fractions of methanol extract (hexane, ethyl acetate, and n-butanol).

Materials and Methods: Phenolic and flavonoid contents were detected using spectrophotometric and colorimetric assays. Antioxidant and anti-inflammatory activities were estimated in-vitro. Anticancer activity of extracts was tested on Hepatocellular carcinoma cell line (HepG2) and breast cancer cell line (MCF7).

Results: The methanolic extract and its ethyl acetate fraction showed higher contents of total phenols and flavonoids. Also, the methanolic extract showed a higher antioxidant activity. The butanolic and ethyl acetate fractions at concentration 500μg/ml yielded a higher percent of inhibition of protein denaturation; 87.9% and 90%, respectively. The ethyl acetate fraction and the methanolic extract showed an obvious anticancer activity against HepG2 and MCF7 (IC50=60±0.24 and 29.4±0.12μg. ml-1) and (IC50=94.7±0.21 and 227±0.48μg. ml-1), respectively. The GC-MS analysis showed that the methanolic extract had 32 compounds whereas the ethyl acetate and butanol fractions contained 40 and 36 compounds, respectively.

Conclusion: Flaxseed contains different biologically active compounds that have been found to possess various activities, which can protect the human body against several diseases.

Keywords:

Flavonoid, flaxseed, hemolysis-assay, HepG2, MCF7, phenolic compounds.

Affiliation:

Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Warraq Al Hadar, Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Warraq Al Hadar, Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Warraq Al Hadar, Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Warraq Al Hadar, Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Warraq Al Hadar



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